研究業績

2014

T. Hara, D. Kashihara, A. Ichimura, I. Kimura, G. Tsujimoto, and A. Hirasawa.
Role of free fatty acid receptors in the regulation of energy metabolism. Biochim. Biophys. Acta, 1841(9):1292–1300, Sep 2014.
http://www.ncbi.nlm.nih.gov/pubmed/24923869

M. Kaneda, R. Misu, H. Ohno, A. Hirasawa, N. Ieda, Y. Uenoyama, H. Tsukamura, K. Maeda, S. Oishi, and N. Fujii.
Design and synthesis of fluorescent probes for GPR54. Bioorg. Med. Chem., 22(13):3325–3330, Jul 2014.
http://www.ncbi.nlm.nih.gov/pubmed/24857775

Sanchez-Reyes, O. B, Romero-Avila, M. T, Castillo-Badillo, J. A, Takei, Y, Hirasawa, A, Tsujimoto, G, Villalobos-Molina, R and Garcia-Sainz, J. A
Free fatty acids and protein kinase C activation induce GPR120 (free fatty acid receptor 4) phosphorylation
Eur. J. Pharmacol. 2014 Jan;723:368–374.
http://www.ncbi.nlm.nih.gov/pubmed/24239485

T. Hara, A. Ichimura, and A. Hirasawa.
Therapeutic role and ligands of medium- to long-chain Fatty Acid receptors. Front Endocrinol (Lausanne), 5:83, 2014.
http://www.ncbi.nlm.nih.gov/pubmed/24917851

A. Ichimura, T. Hara, and A. Hirasawa.
Regulation of Energy Homeostasis via GPR120. Front Endocrinol (Lausanne), 5:111, 2014.
http://www.ncbi.nlm.nih.gov/pubmed/25071726

2013

Hou, Z, Oishi, S, Suzuki, Y, Kure, T, Nakanishi, I, Hirasawa, A, Tsujimoto, G, Ohno, H and Fujii, N
Diversity-oriented synthesis of pyrazolo[4,3-b]indoles by gold-catalysed three-component annulation: application to the development of a new class of CK2 inhibitors
Org. Biomol. Chem. 2013 May;11(20):3288–3296.
http://www.ncbi.nlm.nih.gov/pubmed/23535832

Takeuchi, M, Hirasawa, A, Hara, T, Kimura, I, Hirano, T, Suzuki, T, Miyata, N, Awaji, T, Ishiguro, M and Tsujimoto, G
FFA1-selective agonistic activity based on docking simulation using FFA1 and GPR120 homology models
Br. J. Pharmacol. 2013 Apr;168(7):1570–1583.
http://www.ncbi.nlm.nih.gov/pubmed/22639973

2012

Hara, T, Hirasawa, A, Ichimura, A, Kimura, I and Tsujimoto, G
Physiological functions of fatty acid receptors and their therapeutic potential
Nippon Yakurigaku Zasshi 2012 Dec;140(6):275–279.
http://www.ncbi.nlm.nih.gov/pubmed/23229634

Suzuki, Y, Oishi, S, Takei, Y, Yasue, M, Misu, R, Naoe, S, Hou, Z, Kure, T, Nakanishi, I, Ohno, H, Hirasawa, A, Tsujimoto, G and Fujii, N
Design and synthesis of a novel class of CK2 inhibitors: application of copper- and gold-catalysed cascade reactions for fused nitrogen heterocycles
Org. Biomol. Chem. 2012 Jul;10(25):4907–4915.
http://www.ncbi.nlm.nih.gov/pubmed/22610034

Masuda, R, Oishi, S, Tanahara, N, Ohno, H, Hirasawa, A, Tsujimoto, G, Yano, Y, Matsuzaki, K, Navenot, J. M, Peiper, S. C and Fujii, N
Paradoxical Downregulation of CXC Chemokine Receptor 4 Induced by Polyphemusin II-Derived Antagonists
Bioconjug. Chem. 2012 Jun;23(6):1259–1265.
http://www.ncbi.nlm.nih.gov/pubmed/22486464

Masuda, R, Oishi, S, Tanahara, N, Ohno, H, Hirasawa, A, Tsujimoto, G, Kodama, E, Matsuoka, M and Fujii, N
Development and application of fluorescent SDF-1 derivatives
Future Med Chem 2012 May;4(7):837–844.
http://www.ncbi.nlm.nih.gov/pubmed/22571609

Inoue, D, Kimura, I, Wakabayashi, M, Tsumoto, H, Ozawa, K, Hara, T, Takei, Y, Hirasawa, A, Ishihama, Y and Tsujimoto, G
Short-chain fatty acid receptor GPR41-mediated activation of sympathetic neurons involves synapsin 2b phosphorylation
FEBS Lett. 2012 May;586(10):1547–1554.
http://www.ncbi.nlm.nih.gov/pubmed/22673524

Hou, Z, Nakanishi, I, Kinoshita, T, Takei, Y, Yasue, M, Misu, R, Suzuki, Y, Nakamura, S, Kure, T, Ohno, H, Murata, K, Kitaura, K, Hirasawa, A, Tsujimoto, G, Oishi, S and Fujii, N
Structure-based design of novel potent protein kinase CK2 (CK2) inhibitors with phenyl-azole scaffolds
J. Med. Chem. 2012 Mar;55(6):2899–2903.
http://www.ncbi.nlm.nih.gov/pubmed/22339433

Ichimura, A, Hirasawa, A, Poulain-Godefroy, O, Bonnefond, A, Hara, T, Yengo, L, Kimura, I, Leloire, A, Liu, N, Iida, K, Choquet, H, Besnard, P, Lecoeur, C, Vivequin, S, Ayukawa, K, Takeuchi, M, Ozawa, K, Tauber, M, Maffeis, C, Morandi, A, Buzzetti, R, Elliott, P, Pouta, A, Jarvelin, M. R, Korner, A, Kiess, W, Pigeyre, M, Caiazzo, R, Van Hul, W, Van Gaal, L, Horber, F, Balkau, B, Levy-Marchal, C, Rouskas, K, Kouvatsi, A, Hebebrand, J, Hinney, A, Scherag, A, Pattou, F, Meyre, D, Koshimizu, T. A, Wolowczuk, I, Tsujimoto, G and Froguel, P
Dysfunction of lipid sensor GPR120 leads to obesity in both mouse and human
Nature 2012 Mar;483(7389):350–354.
http://www.ncbi.nlm.nih.gov/pubmed/22343897

2011

Kinoshita, T, Sekiguchi, Y, Fukada, H, Nakaniwa, T, Tada, T, Nakamura, S, Kitaura, K, Ohno, H, Suzuki, Y, Hirasawa, A, Nakanishi, I and Tsujimoto, G
A detailed thermodynamic profile of cyclopentyl and isopropyl derivatives binding to CK2 kinase
Mol. Cell. Biochem. 2011 Oct;356(1-2):97–105.
http://www.ncbi.nlm.nih.gov/pubmed/21735094

Hara, T, Hirasawa, A, Ichimura, A, Kimura, I and Tsujimoto, G
Free fatty acid receptors FFAR1 and GPR120 as novel therapeutic targets for metabolic disorders
J Pharm Sci 2011 Sep;100(9):3594–3601.
http://www.ncbi.nlm.nih.gov/pubmed/21618241

Kimura, I, Inoue, D, Maeda, T, Hara, T, Ichimura, A, Miyauchi, S, Kobayashi, M, Hirasawa, A and Tsujimoto, G
Short-chain fatty acids and ketones directly regulate sympathetic nervous system via G protein-coupled receptor 41 (GPR41)
Proc. Natl. Acad. Sci. U.S.A. 2011 May;108(19):8030–8035.
http://www.ncbi.nlm.nih.gov/pubmed/21518883

2010

Sun, Q, Hirasawa, A, Hara, T, Kimura, I, Adachi, T, Awaji, T, Ishiguro, M, Suzuki, T, Miyata, N and Tsujimoto, G
Structure-activity relationships of GPR120 agonists based on a docking simulation
Mol. Pharmacol. 2010 Nov;78(5):804–810.
http://www.ncbi.nlm.nih.gov/pubmed/20685848

Kimura, I, Nakayama, Y, Konishi, M, Kobayashi, T, Mori, M, Ito, M, Hirasawa, A, Tsujimoto, G, Ohta, M, Itoh, N and Fujimoto, M
Neuferricin, a novel extracellular heme-binding protein, promotes neurogenesis
J. Neurochem. 2010 Mar;112(5):1156–1167.
http://www.ncbi.nlm.nih.gov/pubmed/19968755

Koshimizu, T. A, Tsuchiya, H, Tsuda, H, Fujiwara, Y, Shibata, K, Hirasawa, A, Tsujimoto, G and Fujimura, A
Inhibition of heat shock protein 90 attenuates adenylate cyclase sensitization after chronic morphine treatment
Biochem. Biophys. Res. Commun. 2010 Feb;392(4):603–607.
http://www.ncbi.nlm.nih.gov/pubmed/20100459

Miyauchi, S, Hirasawa, A, Ichimura, A, Hara, T and Tsujimoto, G
New frontiers in gut nutrient sensor research: free fatty acid sensing in the gastrointestinal tract
J. Pharmacol. Sci. 2010;112(1):19–24.
http://www.ncbi.nlm.nih.gov/pubmed/20093784

2009

Ichimura, A, Hirasawa, A, Hara, T and Tsujimoto, G
Free fatty acid receptors act as nutrient sensors to regulate energy homeostasis
Prostaglandins Other Lipid Mediat. 2009 Sep;89(3-4):82–88.
http://www.ncbi.nlm.nih.gov/pubmed/19460454

Hara, T, Hirasawa, A, Sun, Q, Sadakane, K, Itsubo, C, Iga, T, Adachi, T, Koshimizu, T. A, Hashimoto, T, Asakawa, Y and Tsujimoto, G
Novel selective ligands for free fatty acid receptors GPR120 and GPR40
Naunyn Schmiedebergs Arch. Pharmacol. 2009 Sep;380(3):247–255.
http://www.ncbi.nlm.nih.gov/pubmed/19471906

Sekiguchi, Y, Nakaniwa, T, Kinoshita, T, Nakanishi, I, Kitaura, K, Hirasawa, A, Tsujimoto, G and Tada, T
Structural insight into human CK2alpha in complex with the potent inhibitor ellagic acid
Bioorg. Med. Chem. Lett. 2009 Jun;19(11):2920–2923.
http://www.ncbi.nlm.nih.gov/pubmed/19414254

Miyauchi, S, Hirasawa, A, Iga, T, Liu, N, Itsubo, C, Sadakane, K, Hara, T and Tsujimoto, G
Distribution and regulation of protein expression of the free fatty acid receptor GPR120
Naunyn Schmiedebergs Arch. Pharmacol. 2009 Apr;379(4):427–434.
http://www.ncbi.nlm.nih.gov/pubmed/19145429

Kimura, I, Konishi, M, Asaki, T, Furukawa, N, Ukai, K, Mori, M, Hirasawa, A, Tsujimoto, G, Ohta, M, Itoh, N and Fujimoto, M
Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade
Biochem. Biophys. Res. Commun. 2009 Mar;381(1):75–80.
http://www.ncbi.nlm.nih.gov/pubmed/19351598

Nakaniwa, T, Kinoshita, T, Sekiguchi, Y, Tada, T, Nakanishi, I, Kitaura, K, Suzuki, Y, Ohno, H, Hirasawa, A and Tsujimoto, G
Structure of human protein kinase CK2 alpha 2 with a potent indazole-derivative inhibitor
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2009 Feb;65(Pt 2):75–79.
http://www.ncbi.nlm.nih.gov/pubmed/19193990

Hara, T, Hirasawa, A, Sun, Q, Koshimizu, T. A, Itsubo, C, Sadakane, K, Awaji, T and Tsujimoto, G
Flow cytometry-based binding assay for GPR40 (FFAR1; free fatty acid receptor 1)
Mol. Pharmacol. 2009 Jan;75(1):85–91.
http://www.ncbi.nlm.nih.gov/pubmed/18927207

2008

Suzuki, T, Igari, S, Hirasawa, A, Hata, M, Ishiguro, M, Fujieda, H, Itoh, Y, Hirano, T, Nakagawa, H, Ogura, M, Makishima, M, Tsujimoto, G and Miyata, N
Identification of G protein-coupled receptor 120-selective agonists derived from PPARgamma agonists
J. Med. Chem. 2008 Dec;51(23):7640–7644.
http://www.ncbi.nlm.nih.gov/pubmed/19007110

Hirasawa, A, Hara, T, Katsuma, S, Adachi, T and Tsujimoto, G
Free fatty acid receptors and drug discovery
Biol. Pharm. Bull. 2008 Oct;31(10):1847–1851.
http://www.ncbi.nlm.nih.gov/pubmed/18827341

Suzuki, Y, Cluzeau, J, Hara, T, Hirasawa, A, Tsujimoto, G, Oishi, S, Ohno, H and Fujii, N
Structure-activity relationships of pyrazine-based CK2 inhibitors: synthesis and evaluation of 2,6-disubstituted pyrazines and 4,6-disubstituted pyrimidines
Arch. Pharm. (Weinheim) 2008 Sep;341(9):554–561.
http://www.ncbi.nlm.nih.gov/pubmed/18763715

Tanaka, T, Katsuma, S, Adachi, T, Koshimizu, T. A, Hirasawa, A and Tsujimoto, G
Free fatty acids induce cholecystokinin secretion through GPR120
Naunyn Schmiedebergs Arch. Pharmacol. 2008 Jun;377(4-6):523–527.
http://www.ncbi.nlm.nih.gov/pubmed/17972064

Tanaka, T, Yano, T, Adachi, T, Koshimizu, T. A, Hirasawa, A and Tsujimoto, G
Cloning and characterization of the rat free fatty acid receptor GPR120: in vivo effect of the natural ligand on GLP-1 secretion and proliferation of pancreatic beta cells
Naunyn Schmiedebergs Arch. Pharmacol. 2008 Jun;377(4-6):515–522.
http://www.ncbi.nlm.nih.gov/pubmed/18320172

Oishi, S, Masuda, R, Evans, B, Ueda, S, Goto, Y, Ohno, H, Hirasawa, A, Tsujimoto, G, Wang, Z, Peiper, S. C, Naito, T, Kodama, E, Matsuoka, M and Fujii, N
Synthesis and application of fluorescein- and biotin-labeled molecular probes for the chemokine receptor CXCR4
Chembiochem 2008 May;9(7):1154–1158.
http://www.ncbi.nlm.nih.gov/pubmed/18412193

Adachi, T, Yanaka, H, Kanai, H, Nozaki, M, Takahara, Y, Tsuda, M, Jonouchi, T, Tsuda, K, Hirasawa, A and Tsujimoto, G
Administration of perilla oil coated with Calshell increases glucagon-like peptide secretion
Biol. Pharm. Bull. 2008 May;31(5):1021–1023.
http://www.ncbi.nlm.nih.gov/pubmed/18451539

Ichimura, A, Kadowaki, T, Narukawa, K, Togiya, K, Hirasawa, A and Tsujimoto, G
In silico approach to identify the expression of the undiscovered molecules from microarray public database: identification of odorant receptors expressed in non-olfactory tissues
Naunyn Schmiedebergs Arch. Pharmacol. 2008 Apr;377(2):159–165.
http://www.ncbi.nlm.nih.gov/pubmed/18228002

Hirasawa, A, Itsubo, C, Sadakane, K, Hara, T, Shinagawa, S, Koga, H, Nose, H, Koshimizu, T. A and Tsujimoto, G
Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1)
Biochem. Biophys. Res. Commun. 2008 Jan;365(1):22–28.
http://www.ncbi.nlm.nih.gov/pubmed/17980148

Zhang, H, Ohmi, K, Hirasawa, A, Enosawa, S, Hara, Y, Tamura, A and Tsujimoto, G
Immunological tolerance-related genes in a spontaneous tolerant model of rat liver transplantation explored by suppression subtractive hybridization
Cell Transplant 2008;17(1-2):195–201.
http://www.ncbi.nlm.nih.gov/pubmed/18468250

2007

Gotoh, C, Hong, Y. H, Iga, T, Hishikawa, D, Suzuki, Y, Song, S. H, Choi, K. C, Adachi, T, Hirasawa, A, Tsujimoto, G, Sasaki, S and Roh, S. G
The regulation of adipogenesis through GPR120
Biochem. Biophys. Res. Commun. 2007 Mar;354(2):591–597.
http://www.ncbi.nlm.nih.gov/pubmed/17250804

Ruike, Y, Katsuma, S, Hirasawa, A and Tsujimoto, G
Glucocorticoid-induced alternative promoter usage for a novel 5′ variant of granzyme A
J. Hum. Genet. 2007;52(2):172–178.
http://www.ncbi.nlm.nih.gov/pubmed/17180578

2006

Fukunaga, S, Setoguchi, S, Hirasawa, A and Tsujimoto, G
Monitoring ligand-mediated internalization of G protein-coupled receptor as a novel pharmacological approach
Life Sci. 2006 Dec;80(1):17–23.
http://www.ncbi.nlm.nih.gov/pubmed/16978657

Adachi, T, Tanaka, T, Takemoto, K, Koshimizu, T. A, Hirasawa, A and Tsujimoto, G
Free fatty acids administered into the colon promote the secretion of glucagon-like peptide-1 and insulin
Biochem. Biophys. Res. Commun. 2006 Feb;340(1):332–337.
http://www.ncbi.nlm.nih.gov/pubmed/16356474

Herath, C. B, Ishiwata, H, Shiojima, S, Kadowaki, T, Katsuma, S, Ushizawa, K, Imai, K, Takahashi, T, Hirasawa, A, Takahashi, S, Izaike, Y, Tsujimoto, G and Hashizume, K
Developmental aberrations of liver gene expression in bovine fetuses derived from somatic cell nuclear transplantation
Cloning Stem Cells 2006;8(2):79–95.
http://www.ncbi.nlm.nih.gov/pubmed/16776600

2005

Katsuma, S, Hatae, N, Yano, T, Ruike, Y, Kimura, M, Hirasawa, A and Tsujimoto, G
Free fatty acids inhibit serum deprivation-induced apoptosis through GPR120 in a murine enteroendocrine cell line STC-1
J. Biol. Chem. 2005 May;280(20):19507–19515.
http://www.ncbi.nlm.nih.gov/pubmed/15774482

Katsuma, S, Ruike, Y, Yano, T, Kimura, M, Hirasawa, A and Tsujimoto, G
Transcriptional regulation of connective tissue growth factor by sphingosine 1-phosphate in rat cultured mesangial cells
FEBS Lett. 2005 May;579(12):2576–2582.
http://www.ncbi.nlm.nih.gov/pubmed/15862293

Yamada, M, Katsuma, S, Adachi, T, Hirasawa, A, Shiojima, S, Kadowaki, T, Okuno, Y, Koshimizu, T. A, Fujii, S, Sekiya, Y, Miyamoto, Y, Tamura, M, Yumura, W, Nihei, H, Kobayashi, M and Tsujimoto, G
Inhibition of protein kinase CK2 prevents the progression of glomerulonephritis
Proc. Natl. Acad. Sci. U.S.A. 2005 May;102(21):7736–7741.
http://www.ncbi.nlm.nih.gov/pubmed/15897466

Katsuma, S, Hirasawa, A and Tsujimoto, G
Bile acids promote glucagon-like peptide-1 secretion through TGR5 in a murine enteroendocrine cell line STC-1
Biochem. Biophys. Res. Commun. 2005 Apr;329(1):386–390.
http://www.ncbi.nlm.nih.gov/pubmed/15721318

Hirasawa, A, Tsumaya, K, Awaji, T, Katsuma, S, Adachi, T, Yamada, M, Sugimoto, Y, Miyazaki, S and Tsujimoto, G
Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120
Nat. Med. 2005 Jan;11(1):90–94.
http://www.ncbi.nlm.nih.gov/pubmed/15619630

2004

Ushizawa, K, Herath, C. B, Kaneyama, K, Shiojima, S, Hirasawa, A, Takahashi, T, Imai, K, Ochiai, K, Tokunaga, T, Tsunoda, Y, Tsujimoto, G and Hashizume, K
cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period
Reprod. Biol. Endocrinol. 2004 Nov;2:77.
http://www.ncbi.nlm.nih.gov/pubmed/15560851

Izumi, Y, Izumiya, Y, Shiota, M, Yukimura, T, Shiojima, S, Yamada, M, Kadowaki, T, Katsuma, S, Hirasawa, A, Tsujimoto, G and Iwao, H
Gene expression profile in experimental mesangial proliferative glomerulonephritis
J. Pharmacol. Sci. 2004 Sep;96(1):91–94.
http://www.ncbi.nlm.nih.gov/pubmed/15351794

Koshimizu, T. A, Tsujimoto, G, Hirasawa, A, Kitagawa, Y and Tanoue, A
Carvedilol selectively inhibits oscillatory intracellular calcium changes evoked by human alpha1D- and alpha1B-adrenergic receptors
Cardiovasc. Res. 2004 Sep;63(4):662–672.
http://www.ncbi.nlm.nih.gov/pubmed/15306222

Herath, C. B, Shiojima, S, Ishiwata, H, Katsuma, S, Kadowaki, T, Ushizawa, K, Imai, K, Takahashi, T, Hirasawa, A, Tsujimoto, G and Hashizume, K
Pregnancy-associated changes in genome-wide gene expression profiles in the liver of cow throughout pregnancy
Biochem. Biophys. Res. Commun. 2004 Jan;313(3):666–680.
http://www.ncbi.nlm.nih.gov/pubmed/14697243

2003

Yamada, M, Hirasawa, A, Shiojima, S and Tsujimoto, G
Granzyme A mediates glucocorticoid-induced apoptosis in leukemia cells
FASEB J. 2003 Sep;17(12):1712–1714.
http://www.ncbi.nlm.nih.gov/pubmed/12958185

Fukuhara, Y, Hirasawa, A, Li, X. K, Kawasaki, M, Fujino, M, Funeshima, N, Katsuma, S, Shiojima, S, Yamada, M, Okuyama, T, Suzuki, S and Tsujimoto, G
Gene expression profile in the regenerating rat liver after partial hepatectomy
J. Hepatol. 2003 Jun;38(6):784–792.
http://www.ncbi.nlm.nih.gov/pubmed/12763372

Koshimizu, T. A, Tanoue, A, Hirasawa, A, Yamauchi, J and Tsujimoto, G
Recent advances in alpha1-adrenoceptor pharmacology
Pharmacol. Ther. 2003 May;98(2):235–244.
http://www.ncbi.nlm.nih.gov/pubmed/12725871

Ishiwata, H, Katsuma, S, Kizaki, K, Patel, O. V, Nakano, H, Takahashi, T, Imai, K, Hirasawa, A, Shiojima, S, Ikawa, H, Suzuki, Y, Tsujimoto, G, Izaike, Y, Todoroki, J and Hashizume, K
Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray
Mol. Reprod. Dev. 2003 May;65(1):9–18.
http://www.ncbi.nlm.nih.gov/pubmed/12658628

Richardson, J, Chatwin, H, Hirasawa, A, Tsujimoto, G and Evans, P. D
Agonist-specific coupling of a cloned human alpha1A-adrenoceptor to different second messenger pathways
Naunyn Schmiedebergs Arch. Pharmacol. 2003 Apr;367(4):333–341.
http://www.ncbi.nlm.nih.gov/pubmed/12690424

Nakamura, T, Shiojima, S, Hirai, Y, Iwama, T, Tsuruzoe, N, Hirasawa, A, Katsuma, S and Tsujimoto, G
Temporal gene expression changes during adipogenesis in human mesenchymal stem cells
Biochem. Biophys. Res. Commun. 2003 Mar;303(1):306–312.
http://www.ncbi.nlm.nih.gov/pubmed/12646203

Katsuma, S, Hada, Y, Shiojima, S, Hirasawa, A, Tanoue, A, Takagaki, K, Ohgi, T, Yano, J and Tsujimoto, G
Transcriptional profiling of gene expression patterns during sphingosine 1-phosphate-induced mesangial cell proliferation
Biochem. Biophys. Res. Commun. 2003 Jan;300(2):577–584.
http://www.ncbi.nlm.nih.gov/pubmed/12504122

Shibata, K, Katsuma, S, Koshimizu, T, Shinoura, H, Hirasawa, A, Tanoue, A and Tsujimoto, G
alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP-dependent mechanism involving p27Kip1
J. Biol. Chem. 2003 Jan;278(1):672–678.
http://www.ncbi.nlm.nih.gov/pubmed/12409310

2002

Katsuma, S, Hada, Y, Ueda, T, Shiojima, S, Hirasawa, A, Tanoue, A, Takagaki, K, Ohgi, T, Yano, J and Tsujimoto, G
Signalling mechanisms in sphingosine 1-phosphate-promoted mesangial cell proliferation
Genes Cells 2002 Dec;7(12):1217–1230.
http://www.ncbi.nlm.nih.gov/pubmed/12485162

Shinoura, H, Shibata, K, Hirasawa, A, Tanoue, A, Hashimoto, K and Tsujimoto, G
Key amino acids for differential coupling of alpha1-adrenergic receptor subtypes to Gs
Biochem. Biophys. Res. Commun. 2002 Nov;299(1):142–147.
http://www.ncbi.nlm.nih.gov/pubmed/12435400

Yamauchi, J, Miyamoto, Y, Kokubu, H, Nishii, H, Okamoto, M, Sugawara, Y, Hirasawa, A, Tsujimoto, G and Itoh, H
Endothelin suppresses cell migration via the JNK signaling pathway in a manner dependent upon Src kinase, Rac1, and Cdc42
FEBS Lett. 2002 Sep;527(1-3):284–288.
http://www.ncbi.nlm.nih.gov/pubmed/12220675

Katsuma, S, Shiojima, S, Hirasawa, A, Takagaki, K, Kaminishi, Y, Koba, M, Hagidai, Y, Murai, M, Ohgi, T, Yano, J and Tsujimoto, G
Global analysis of differentially expressed genes during progression of calcium oxalate nephrolithiasis
Biochem. Biophys. Res. Commun. 2002 Aug;296(3):544–552.
http://www.ncbi.nlm.nih.gov/pubmed/12176015

Yamauchi, J, Hirasawa, A, Miyamoto, Y, Kokubu, H, Nishii, H, Okamoto, M, Sugawara, Y, Tsujimoto, G and Itoh, H
Role of Dbl’s big sister in the anti-mitogenic pathway from alpha1B-adrenergic receptor to c-Jun N-terminal kinase
Biochem. Biophys. Res. Commun. 2002 Aug;296(1):85–92.
http://www.ncbi.nlm.nih.gov/pubmed/12147231

Koshimizu, T. A, Yamauchi, J, Hirasawa, A, Tanoue, A and Tsujimoto, G
Recent progress in alpha 1-adrenoceptor pharmacology
Biol. Pharm. Bull. 2002 Apr;25(4):401–408.
http://www.ncbi.nlm.nih.gov/pubmed/11995914

Sugawara, T, Hirasawa, A, Hashimoto, K and Tsujimoto, G
Differences in the subcellular localization of alpha1-adrenoceptor subtypes can affect the subtype selectivity of drugs in a study with the fluorescent ligand BODIPY FL-prazosin
Life Sci. 2002 Mar;70(18):2113–2124.
http://www.ncbi.nlm.nih.gov/pubmed/12002804

Yoshida, N, Sugiyama, M, Tanoue, A, Hirasawa, A, Saito, H and Tsujimoto, G
Determination of a novel haplotype of beta2-adrenergic receptor in the Japanese population by the combination of the electronic microchip assay using the NanoChip system with allele-specific PCR
Drug Metab. Pharmacokinet. 2002;17(6):532–539.
http://www.ncbi.nlm.nih.gov/pubmed/15618708

Hashizume, K, Ishiwata, H, Kizaki, K, Yamada, O, Takahashi, T, Imai, K, Patel, O. V, Akagi, S, Shimizu, M, Takahashi, S, Katsuma, S, Shiojima, S, Hirasawa, A, Tsujimoto, G, Todoroki, J and Izaike, Y
Implantation and placental development in somatic cell clone recipient cows
Cloning Stem Cells 2002;4(3):197–209.
http://www.ncbi.nlm.nih.gov/pubmed/12398801

Yoshida, N, Nishimaki, Y, Sugiyama, M, Abe, T, Tatsumi, T, Tanoue, A, Hirasawa, A and Tsujimoto, G
SNP genotyping in the beta(2)-adrenergic receptor by electronic microchip assay, DHPLC, and direct sequencing
J. Hum. Genet. 2002;47(9):500–503.
http://www.ncbi.nlm.nih.gov/pubmed/12202992

Katsuma, S, Shiojima, S, Hirasawa, A, Suzuki, Y, Ikawa, H, Takagaki, K, Kaminishi, Y, Murai, M, Ohgi, T, Yano, J and Tsujimoto, G
Functional genomic search of G-protein-coupled receptors using microarrays with normalized cDNA library
Meth. Enzymol. 2002;345:585–600.
http://www.ncbi.nlm.nih.gov/pubmed/11665641

2001

Awaji, T, Hirasawa, A, Shirakawa, H, Tsujimoto, G and Miyazaki, S
Novel green fluorescent protein-based ratiometric indicators for monitoring pH in defined intracellular microdomains
Biochem. Biophys. Res. Commun. 2001 Nov;289(2):457–462.
http://www.ncbi.nlm.nih.gov/pubmed/11716495

Yamauchi, J, Itoh, H, Shinoura, H, Miyamoto, Y, Tsumaya, K, Hirasawa, A, Kaziro, Y and Tsujimoto, G
Galphaq-dependent activation of mitogen-activated protein kinase kinase 4/c-Jun N-terminal kinase cascade
Biochem. Biophys. Res. Commun. 2001 Nov;288(5):1087–1094.
http://www.ncbi.nlm.nih.gov/pubmed/11700022

Katsuma, S, Nishi, K, Tanigawara, K, Ikawa, H, Shiojima, S, Takagaki, K, Kaminishi, Y, Suzuki, Y, Hirasawa, A, Ohgi, T, Yano, J, Murakami, Y and Tsujimoto, G
Molecular monitoring of bleomycin-induced pulmonary fibrosis by cDNA microarray-based gene expression profiling
Biochem. Biophys. Res. Commun. 2001 Nov;288(4):747–751.
http://www.ncbi.nlm.nih.gov/pubmed/11688970

Tsujimoto, G, Katsuma, S, Shiojima, S, Hirasawa, A and Tanoue, A
Functional genomic research of alpha 1-adrenoceptors
J. Cardiovasc. Pharmacol. 2001 Oct;38 Suppl 1:1–5.
http://www.ncbi.nlm.nih.gov/pubmed/11811351

Takahara, A, Hirasawa, A, Dohmoto, H, Shoji, M, Yoshimoto, R, Sugiyama, A and Hashimoto, K
In vivo antiarrhythmic profile of AP-792 assessed in different canine arrhythmia models
Jpn. J. Pharmacol. 2001 Sep;87(1):21–26.
http://www.ncbi.nlm.nih.gov/pubmed/11676194

Yamauchi, J, Hirasawa, A, Miyamoto, Y, Itoh, H and Tsujimoto, G
Beta2-adrenergic receptor/cyclic adenosine monophosphate (cAMP) leads to JNK activation through Rho family small GTPases
Biochem. Biophys. Res. Commun. 2001 Jun;284(5):1199–1203.
http://www.ncbi.nlm.nih.gov/pubmed/11414711

Hirasawa, A, Awaji, T, Xu, Z, Shinoura, H and Tsujimoto, G
Regulation of subcellular localization of alpha1-adrenoceptor subtypes
Life Sci. 2001 Apr;68(19-20):2259–2267.
http://www.ncbi.nlm.nih.gov/pubmed/11358335

Yamauchi, J, Itoh, H, Shinoura, H, Miyamoto, Y, Hirasawa, A, Kaziro, Y and Tsujimoto, G
Involvement of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase in alpha1B-adrenergic receptor/Galphaq-induced inhibition of cell proliferation
Biochem. Biophys. Res. Commun. 2001 Mar;281(4):1019–1023.
http://www.ncbi.nlm.nih.gov/pubmed/11237765

Katsuma, S, Shiojima, S, Hirasawa, A, Suzuki, Y, Takagaki, K, Murai, M, Kaminishi, Y, Hada, Y, Koba, M, Muso, E, Miyawaki, S, Ohgi, T, Yano, J and Tsujimoto, G
Genomic analysis of a mouse model of immunoglobulin A nephropathy reveals an enhanced PDGF-EDG5 cascade
Pharmacogenomics J. 2001;1(3):211–217.
http://www.ncbi.nlm.nih.gov/pubmed/11908758

2000

Homma, N, Hirasawa, A, Shibata, K, Hashimito, K and Tsujimoto, G
Both alpha(1A)- and alpha(1B)-adrenergic receptor subtypes couple to the transient outward current (I(To)) in rat ventricular myocytes
Br. J. Pharmacol. 2000 Mar;129(6):1113–1120.
http://www.ncbi.nlm.nih.gov/pubmed/10725259

Shinoura, H, Take, H, Itoh, S, Hirasawa, A, Inoue, K, Ohno, Y, Hashimoto, K and Tsujimoto, G
Key amino acids of vasopressin V1a receptor responsible for the species difference in the affinity of OPC-21268
FEBS Lett. 2000 Jan;466(2-3):255–258.
http://www.ncbi.nlm.nih.gov/pubmed/10682838

1999

Xu, Z, Hirasawa, A, Shinoura, H and Tsujimoto, G
Interaction of the alpha(1B)-adrenergic receptor with gC1q-R, a multifunctional protein
J. Biol. Chem. 1999 Jul;274(30):21149–21154.
http://www.ncbi.nlm.nih.gov/pubmed/10409668

1998

Awaji, T, Hirasawa, A, Kataoka, M, Shinoura, H, Nakayama, Y, Sugawara, T, Izumi, S and Tsujimoto, G
Real-time optical monitoring of ligand-mediated internalization of alpha1b-adrenoceptor with green fluorescent protein
Mol. Endocrinol. 1998 Aug;12(8):1099–1111.
http://www.ncbi.nlm.nih.gov/pubmed/9717836

Take, H, Shibata, K, Awaji, T, Hirasawa, A, Ikegaki, I, Asano, T, Takada, T and Tsujimoto, G
Vascular alpha1-adrenoceptor subtype selectivity and alpha1-blocker-induced orthostatic hypotension
Jpn. J. Pharmacol. 1998 May;77(1):61–70.
http://www.ncbi.nlm.nih.gov/pubmed/9639061

Hirasawa, A, Awaji, T, Sugawara, T, Tsujimoto, A and Tsujimoto, G
Differential mechanism for the cell surface sorting and agonist-promoted internalization of the alpha1B-adrenoceptor
Br. J. Pharmacol. 1998 May;124(1):55–62.
http://www.ncbi.nlm.nih.gov/pubmed/9630343

Shibata, K, Hirasawa, A, Foglar, R, Ogawa, S and Tsujimoto, G
Effects of quinidine and verapamil on human cardiovascular alpha1-adrenoceptors
Circulation 1998 Apr;97(13):1227–1230.
http://www.ncbi.nlm.nih.gov/pubmed/9570190

Tsujimoto, G, Hirasawa, A, Sugawara, T and Awaji, T
Subtype-specific differences in subcellular localization and chlorethylclonidine inactivation of alpha1-adrenoceptors
Life Sci. 1998;62(17-18):1567–1571.
http://www.ncbi.nlm.nih.gov/pubmed/9585137

1997

Hirasawa, A, Sugawara, T, Awaji, T, Tsumaya, K, Ito, H and Tsujimoto, G
Subtype-specific differences in subcellular localization of alpha1-adrenoceptors: chlorethylclonidine preferentially alkylates the accessible cell surface alpha1-adrenoceptors irrespective of the subtype
Mol. Pharmacol. 1997 Nov;52(5):764–770.
http://www.ncbi.nlm.nih.gov/pubmed/9351966

1996

Hirasawa, A, Athauda, S. B and Takahashi, K
Purification and characterization of turtle pepsinogen and pepsin
J. Biochem. 1996 Aug;120(2):407–414.
http://www.ncbi.nlm.nih.gov/pubmed/8889828

Awaji, T, Izumi, S, Horie, K, Hirasawa, A and Tsujimoto, G
Alpha 1b-adrenoceptor-mediated calcium oscillation is specific for the S phase in cell cycle and dependent on the extracellular calcium
Biochem. Biophys. Res. Commun. 1996 Jul;224(1):80–86.
http://www.ncbi.nlm.nih.gov/pubmed/8694837

Shibata, K, Hirasawa, A, Moriyama, N, Kawabe, K, Ogawa, S and Tsujimoto, G
Alpha 1a-adrenoceptor polymorphism: pharmacological characterization and association with benign prostatic hypertrophy
Br. J. Pharmacol. 1996 Jul;118(6):1403–1408.
http://www.ncbi.nlm.nih.gov/pubmed/8832064

Hirasawa, A, Tsumaya, K, Awaji, T, Shibata, K, Homma, N, Shinomiya, T and Tsujimoto, G
Flow cytometry analysis of alpha1-adrenoceptor subtypes
FEBS Lett. 1996 May;386(2-3):141–148.
http://www.ncbi.nlm.nih.gov/pubmed/8647269

Yazawa, H, Hirasawa, A, Horie, K, Saita, Y, Iida, E, Honda, K and Tsujimoto, G
Oxytocin receptors expressed and coupled to Ca2+ signalling in a human vascular smooth muscle cell line
Br. J. Pharmacol. 1996 Mar;117(5):799–804.
http://www.ncbi.nlm.nih.gov/pubmed/8851493

1995

Hirasawa, A, Awaji, T and Tsujimoto, G
[Flow cytometry and laser scanning confocal microscopy analysis of the receptor distribution]
Nippon Yakurigaku Zasshi 1995 Oct;106(4):255–261.
http://www.ncbi.nlm.nih.gov/pubmed/8537072

Kato, Y, Igarashi, N, Hirasawa, A, Tsujimoto, G and Kobayashi, M
Distribution and developmental changes in vasopressin V2 receptor mRNA in rat brain
Differentiation 1995 Oct;59(3):163–169.
http://www.ncbi.nlm.nih.gov/pubmed/7589900

Esbenshade, T. A, Hirasawa, A, Tsujimoto, G, Tanaka, T, Yano, J, Minneman, K. P and Murphy, T. J
Cloning of the human alpha 1d-adrenergic receptor and inducible expression of three human subtypes in SK-N-MC cells
Mol. Pharmacol. 1995 May;47(5):977–985.
http://www.ncbi.nlm.nih.gov/pubmed/7746284

Hashimoto, K, Haruno, A, Hirasawa, A, Awaji, T, Xue, Y and Wu, Z
Effects of the new class III antiarrhythmic drug MS-551 and d-sotalol on canine coronary ligation-reperfusion ventricular arrhythmias
Jpn. J. Pharmacol. 1995 May;68(1):1–9.
http://www.ncbi.nlm.nih.gov/pubmed/7494370

Hirasawa, A, Shibata, K, Horie, K, Takei, Y, Obika, K, Tanaka, T, Muramoto, N, Takagaki, K, Yano, J and Tsujimoto, G
Cloning, functional expression and tissue distribution of human alpha 1c-adrenoceptor splice variants
FEBS Lett. 1995 Apr;363(3):256–260.
http://www.ncbi.nlm.nih.gov/pubmed/7737411

Hirasawa, A, Tsujimoto, G, Okuyama, S, Li, X. K, Iwaya, M, Masaki, Y, Yokoi, Y, Nakamura, S, Baba, S and Miyamoto, M
Polymerase chain reaction of the rat sex-determining region of the Y-chromosome and its application to estimating a state of sensitization to minor histocompatibility antigen H-Y
Transplant. Proc. 1995 Apr;27(2):1598–1600.
http://www.ncbi.nlm.nih.gov/pubmed/7725420

Hara, M, Shibata, K, Hirasawa, A, Iwaya, M, Okuyama, S, Tsujimoto, G, Kimura, H and Koga, Y
Microchimerism and lung allograft acceptance
Transplant. Proc. 1995 Apr;27(2):1580–1582.
http://www.ncbi.nlm.nih.gov/pubmed/7725416

Kaneko, H, Iizuka, K, Kokai, Y, Fujimoto, J. I, Hirasawa, A, Tsujimoto, G, Koike, H, Torres-Nagel, N, Hunig, T and Kimura, H
Vaccination with a synthetic T-cell receptor V-region peptide: immunomodulation or vaccination
Transplant. Proc. 1995 Apr;27(2):1539–1542.
http://www.ncbi.nlm.nih.gov/pubmed/7536968

Masaki, Y, Hirasawa, A, Okuyama, S, Tsujimoto, G, Iwaya, M, Li, X. K, Yokoi, Y, Nakamura, S, Baba, S and Miyamoto, M
Microchimerism and heart allograft acceptance
Transplant. Proc. 1995 Feb;27(1):148–150.
http://www.ncbi.nlm.nih.gov/pubmed/7878949

Suzuki, H, Shibata, H, Murakami, M, Sato, A, Naito, M, Ichihara, A, Matsumoto, A, Tsujimoto, G, Hirasawa, A and Horie, K
Modulation of angiotensin II type 1 receptor mRNA expression in human blood cells: comparison of platelets and mononuclear leucocytes
Endocr. J. 1995 Feb;42(1):15–22.
http://www.ncbi.nlm.nih.gov/pubmed/7599693

Foglar, R, Shibata, K, Horie, K, Hirasawa, A and Tsujimoto, G
Use of recombinant alpha 1-adrenoceptors to characterize subtype selectivity of drugs for the treatment of prostatic hypertrophy
Eur. J. Pharmacol. 1995 Jan;288(2):201–207.
http://www.ncbi.nlm.nih.gov/pubmed/7536677

Takahashi, K, Tanji, M, Yakabe, E, Hirasawa, A, Athauda, S. B and Kageyama, T
Non-mammalian vertebrate pepsinogens and pepsins: isolation and characterization
Adv. Exp. Med. Biol. 1995;362:53–65.
http://www.ncbi.nlm.nih.gov/pubmed/8540368

1994

Hirasawa, A, Nakayama, Y, Ishiharada, N, Honda, K, Saito, R, Tsujimoto, G, Takano, Y and Kamiya, H
Evidence for the existence of vasopressin V2 receptor mRNA in rat hippocampus
Biochem. Biophys. Res. Commun. 1994 Dec;205(3):1702–1706.
http://www.ncbi.nlm.nih.gov/pubmed/7811254

Hirasawa, A, Shibata, K, Kotosai, K and Tsujimoto, G
Cloning, functional expression and tissue distribution of human cDNA for the vascular-type vasopressin receptor
Biochem. Biophys. Res. Commun. 1994 Aug;203(1):72–79.
http://www.ncbi.nlm.nih.gov/pubmed/8074728

Horie, K, Hirasawa, A and Tsujimoto, G
The pharmacological profile of cloned and stably expressed alpha 1b-adrenoceptor in CHO cells
Eur. J. Pharmacol. 1994 Aug;268(3):399–407.
http://www.ncbi.nlm.nih.gov/pubmed/7805764

Hirasawa, A, Hashimoto, K and Tsujimoto, G
Distribution and developmental change of vasopressin V1A and V2 receptor mRNA in rats
Eur. J. Pharmacol. 1994 Mar;267(1):71–75.
http://www.ncbi.nlm.nih.gov/pubmed/8206132

1993

Hirasawa, A, Awaji, T, Hosono, M, Haruno, A and Hashimoto, K
Effects of a new forskolin derivative, NKH477, on canine ventricular arrhythmia models
J. Cardiovasc. Pharmacol. 1993 Dec;22(6):847–851.
http://www.ncbi.nlm.nih.gov/pubmed/7509903

Hirasawa, A, Horie, K, Tanaka, T, Takagaki, K, Murai, M, Yano, J and Tsujimoto, G
Cloning, functional expression and tissue distribution of human cDNA for the alpha 1C-adrenergic receptor
Biochem. Biophys. Res. Commun. 1993 Sep;195(2):902–909.
http://www.ncbi.nlm.nih.gov/pubmed/8396931

Horie, K, Hirasawa, A, Masuda, K and Tsujimoto, G
Identification of alpha 1C-adrenergic receptor mRNA in bovine retinal pigment epithelium
Invest. Ophthalmol. Vis. Sci. 1993 Aug;34(9):2769–2775.
http://www.ncbi.nlm.nih.gov/pubmed/8393846

Zhenjiu, W, Awaji, T, Hirasawa, A, Motomura, S and Hashimoto, K
Effects of a new class I antiarrhythmic drug bidisomide on canine ventricular arrhythmia models
Mol. Cell. Biochem. 1993 Feb;119(1-2):159–169.
http://www.ncbi.nlm.nih.gov/pubmed/8455577

1992

Hirasawa, A, Haruno, A, Matsuzaki, T and Hashimoto, K
Effects of a new antiarrhythmic drug, SD-3212, on canine ventricular arrhythmia models
Jpn Heart J 1992 Nov;33(6):851–861.
http://www.ncbi.nlm.nih.gov/pubmed/1299746

1991

Hashimoto, K, Sugiyama, A, Haruno, A, Matsuzaki, T and Hirasawa, A
Effects of a new antiarrhythmic drug TYB-3823 on canine ventricular arrhythmia models
J. Cardiovasc. Pharmacol. 1991 Feb;17(2):336–342.
http://www.ncbi.nlm.nih.gov/pubmed/1709241